Objective To investigate the application effects of the multi-disciplinary team (MDT) scenario simulation teaching mode in the internship stage of medical imaging majors under the background of "new medical education." Methods From January 2023 to May 2023, twenty interns from the Department of Medical Imaging at the First Affiliated Hospital of Fujian Medical University were designated as the experimental group, receiving MDT scenario simulation teaching in addition to conventional internship teaching. Another 20 interns from two other hospitals were randomly selected as the control group, receiving conventional internship teaching. The effectiveness of the internship teaching was evaluated through formative assessments, summative assessments, and questionnaires. Statistical analysis was performed using the Wilcoxon rank sum test, t-test, and Fisher"s exact probability method. Results The average scores of the experimental group interns in the imaging case analysis exam (stages 2 to 5) and the exit exam [(46.8±4.47)-(60.5±3.56) points] were higher than those of the control group interns [(44.5±5.79)-(55.6±5.19) points], and the difference was statistically significant (t=1.41~3.48, P<0.05). There was no significant difference in scores between the case questions of the first two stage exams and the objective questions of the seven assessments (P>0.05). The satisfaction ratings of the experimental group interns (16.6±1.57) were significantly higher than those of the control group (14.95±2.76) (t=2.323, P=0.027). Conclusions The MDT scenario simulation teaching mode under the background of "new medical education" can effectively enhance the clinical MDT capabilities of medical imaging majors, cultivate their teamwork and science popularization abilities, and has been unanimously affirmed by interns and teachers. This mode is conducive to the cultivation of the essential "precision imaging" ability of outstanding physicians in imaging and deserves further promotion.