The gender equality policy has been implemented for 70 years since the founding of the People"s Republic of China. The gender culture is gradually changing, and the concept of mate selection among medical workers born in the 1980s and 1990s also changed. The survey shows that there are still gender differences under the influence of traditional gender culture. Emphasis on affection and autonomous mate selection have become the mainstream, but women are relatively passive in showing love; emphasis on internal and equal choice is prominent, but slope choice still exists; The popularity and professional preference are significant. As the main body of the construction of healthy China, female medical workers are economically independent, and still need equal gender system arrangements and the evolution of gender culture to resolve the contradiction between their pursuit of career development and family happiness.